In Vitro and In Vivo Biology of Recombinant Adenovirus Vectors with E1, E1/E2A, or E1/E4 Deleted
نویسندگان
چکیده
منابع مشابه
Biology of E1-deleted adenovirus vectors in nonhuman primate muscle.
Adenovirus vectors have been studied as vehicles for gene transfer to skeletal muscle, an attractive target for gene therapies for inherited and acquired diseases. In this setting, immune responses to viral proteins and/or transgene products cause inflammation and lead to loss of transgene expression. A few studies in murine models have suggested that the destructive cell-mediated immune respon...
متن کاملTranscription initiation activity of adenovirus left-end sequence in adenovirus vectors with e1 deleted.
We analyzed the transcription initiation activity of the left-end sequence (first 342 bp) of the adenovirus genome in the context of an adenovirus vector with E1 deleted in in vitro and in vivo gene transfer models. While nucleotide sequences 1 to 190 and 1 to 342 showed strong activity in three out of three lung cancer cell lines, nucleotide sequence 1 to 103 showed limited activity in H358, c...
متن کاملProduction and characterization of improved adenovirus vectors with the E1, E2b, and E3 genes deleted.
Adenovirus (Ad)-based vectors have great potential for use in the gene therapy of multiple diseases, both genetic and nongenetic. While capable of transducing both dividing and quiescent cells efficiently, Ad vectors have been limited by a number of problems. Most Ad vectors are engineered such that a transgene replaces the Ad E1a, E1b, and E3 genes; subsequently the replication-defective vecto...
متن کاملInfluence of E1-Deleted Recombinant Adenoviruses on B7.1 and IL-2 Expression in C1498 Cells
Knowing that adenoviral vectors could initiate innate immunity, the ability of E1-deleted recombinant adenovirus (Ad-E1Δ) in induction of B7.1 and IL-2 molecules was studied. Methods: The expression of green fluorescent protein in C1498 cells following transfection of these cells with adenovirus green fluorescent protein vector confirmed the ability of adenovirus vectors in infecting the cells ...
متن کاملA simple method for constructing E1- and E1/E4-deleted recombinant adenoviral vectors.
We previously developed a two-plasmid in vitro ligation method that did not require a recombination step to produce new recombinant E1- or E1/E3-deleted adenoviral vectors. In this study, we have modified the system to improve the simplicity of vector construction and, in addition, to allow for production of an E1/E4-deleted vector.
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1998
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.72.3.2022-2032.1998